![]() ![]() To ascertain whether polymer structure is indicative of mechanical strength, scanning electron microscopy images were also used to study the structural properties of each resin. Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers for the high-throughput intermediate purification and polishing steps of a wide. Typical packing factors for Capto S, Capto Q, and Capto DEAE in different solutions for optimal bed performance where the bed is consolidated at 3060 cm/h Solution Capto Q Capto S Capto DEAE Water 1.10 1.10 1.10 20 ethanol 1.10 10 mM NaCl 1.17 1.17 1.17 20 ethanol with 0.2 M sodium acetate N/A 1. The most mechanically robust resin (Capto Q) had a critical velocity 3.3 times higher than the weakest (Sepharose CL-4B), whilst the DMA technique showed Capto Q to have a slurry deformation rate 8.3 times lower than Sepharose CL-4B. Results show a strong correlation between the two techniques. This technique was conducted on seven resins with varying degrees of mechanical robustness and the results were compared to pressure-flow test results on the same resins. To address these issues, we have developed a dynamic mechanical analysis (DMA) technique that characterises the mechanical properties of resins based on the viscoelasticity of a 1 ml sample of slurry. Cations are separated on cation-exchange resin column and anions on an anion exchange resin. There are a number of drawbacks with this method, including the potential need for several re-packs to achieve the desired packing quality, the impact of wall effects on experimental set up and the quantities of chromatography media and buffers required. Flexible process design due to large operational window of flow rates and bed heights. Efficient industrial-scale purification based on the well-established Capto platform with traditional ligands. Praesto Q90 demonstrates selectivity equal to Q Sepharose Fast Flow and Capto Q. Capto Q XP is designed for capture and intermediate purification of large biomolecules, such as immunoglobulins IgG, IgM, IgA, in downstream processes. Pressure-flow profiles are most commonly used to characterise these properties. Praesto SP (cation) and Praesto Q (anion) chromatography resins are. Mechanical characterisation of agarose-based resins is an important factor in ensuring robust chromatographic performance in the manufacture of biopharmaceuticals. ![]()
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